Prof. Gali Prag
Research work
The lab study mechanisms of ubiquitin signals and their significance in neurodegenerative/neurodevelopment and brain cancer diseases at the molecular / structural level.
We developed and employ a unique bacterial genetic selection system for ubiquitylation to study such brain diseases. Ubiquitin E3-ligases known to be involve in Autism, Angelman and Kaufman syndromes as well as Epilepsy, Parkinson’s and Alzheimer's disease are our currently main targets. We also study the involvement of novel ubiquitin-receptor in amyloid formation.
Areas of interest & scientific knowledge
Brain Disorders Research
Cellular & Molecular Neuroscience
Neurodevelopment and Neurogenetic Research
Specialization
Selected Publications
- Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases. Attali I, Tobelaim WS, Persaud A, Motamedchaboki K, Simpson-Lavy KJ, Mashahreh B, Levin-Kravets O, Keren-Kaplan T, Pilzer I, Kupiec M, Wiener R, Wolf DA, Rotin D, Prag G. EMBO J. 2017 Feb 15;36(4):425-440. doi: 10.15252/embj.201694314.
https://www.embopress.org/doi/full/10.15252/embj.201694314 Also see News & Views at https://www.embopress.org/doi/full/10.15252/embj.201696154 - Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases. Attali I, Tobelaim WS, Persaud A, Motamedchaboki K, Simpson-Lavy KJ, Mashahreh B, Levin-Kravets O, Keren-Kaplan T, Pilzer I, Kupiec M, Wiener R, Wolf DA, Rotin D, Prag G. EMBO J. 2017 Feb 15;36(4):425-440. doi: 10.15252/embj.201694314.
https://www.embopress.org/doi/full/10.15252/embj.201694314 Also see News & Views at https://www.embopress.org/doi/full/10.15252/embj.201696154 - A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G. Nature Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. https://www.nature.com/articles/nmeth.4003 - Structure-based in silico identification of ubiquitin-binding domains provides insights into the ALIX-V:ubiquitin complex and retrovirus budding. Keren-Kaplan T, Attali I, Estrin M, Kuo LS, Farkash E, Jerabek-Willemsen M, Blutraich N, Artzi S, Peri A, Freed EO, Wolfson HJ, Prag G.
EMBO J. 2013 Feb 20;32(4):538-51. https://www.embopress.org/doi/10.1038/emboj.2013.4 - Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria. Keren-Kaplan T, Attali I, Motamedchaboki K, Davis BA, Tanner N, Reshef Y, Laudon E, Kolot M, Levin-Kravets O, Kleifeld O, Glickman M, Horazdovsky BF, Wolf DA, Prag G. EMBO J. 2012 Jan 18;31(2):378-90. doi: 10.1038/emboj.2011.397.
https://www.embopress.org/doi/10.1038/emboj.2011.397 Also see the Highlighted in Nature Methods https://www.nature.com/articles/nmeth.1835
